Figure 2.

Fractions Enriched in Mixed-Linkage Arabinoglucan Were Identified by Oligosaccharide Release and Dye Binding.

(A) RP-HPLC of supernatants from CTAB precipitation of CDTA extracts of AIR cell wall preparations from N. benthamiana leaves shows material eluting from 3 to 12 min in leaves transformed with Pp3c12_24670 (red), but not in the empty negative control vector (blue).

(B) Fractions collected at 30 s intervals between 2.5 and 13 min (fractions 6–23) were tested for fluorescence intensity in the presence of FB28 and the release of tetrasaccharide by E-CELTR, as detected by HPAEC-PAD (Supplemental Figure 5). Stacked bars report percentages of the maximum value, which is set at 100%. Fractions 11 and 12 were also digested with lichenase and no released oligosaccharides were detected.