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. 2018 Apr 19;30(6):1293–1308. doi: 10.1105/tpc.18.00082

Figure 4.

Figure 4.

Pp3c12_24670 Is Active When Expressed in the Native Host.

(A) HPAEC-PAD profiles of E-CELAN hydrolysates of CDTA-soluble fractions of AIR preparations from N. benthamiana leaves transformed with Pp3c12_24670 expression vector (positive control) or 6-d-old protonema from P. patens transformed with Pp3c12_24670 expression vector, P. patens wild type, P. patens transformed with catalytically inactive Pp3c12_24670 expression vector or empty negative control vector, P. patens Pp3c12_24670 knockout lines, or P. patens transformed with mEGFP: Pp3c12_24670 fusion protein expression vector.

(B) Protein gel blots of protein extracted from stable P. patens lines transformed with Pp3c12_24670 or catalytically inactive Pp3c12_24670 expression vectors. Blots probed with antihemagglutinin are shown above the same blot stained with Ponceau S as a loading control. Protein loading per lane was 3.3 and 5.8 μg per lane, respectively.

(C) HPAEC-PAD profiles of E-CELAN hydrolysates from equal masses of never-dried cell walls from 8-d-old protonema of wild type (blue) and Pp3c12_24670 transformed (black) P. patens.

(D) HPAEC-PAD profiles of E-CELAN and no-enzyme control hydrolysates of never-dried cell walls from mature P. patens gametophores with positive control N. benthamiana transformed with Pp3c12_24670.