Table 1. MAPS-SEQ Has Broad Applicability for the Discovery of Essential Genes Functioning in Diverse Biological Pathways.
| Functional Annotation | No. of Genes | Examples of Likely Orthologs Defined by BLAST |
|---|---|---|
| Cell cycle machinery | 41 | APC1, APC2, APC3, APC4, APC6, APC8, CDC20, AUR2, SMC1, SMC2, SMC3, SMC4, POLA, POLD, POLE |
| General transcription | 9 | POLII, SPT16, TFIIH, SPT6 |
| Splicing | 4 | SRPK, PRP4, PRP8 |
| Translation | 3 | GTS2, DOH1, TSK2 |
| Protein modification | 3 | UBC12, SENP7, CLPP4 |
| Stress/protein folding | 9 | IRE1, HSF, CCT1, SNF1 |
| Glycosylation/secretion | 11 | UDP-galactose and -rhamnose epimerase, UDP-sugar transport, PGI, NSF |
| Transporters | 3 | Magnesium transporter, sulfate transporter |
| Other/unknown | 19 | GIF3, DUO3, clathrin, NUP205, Exportin-2, MIDASIN, BSL1 |
Classes of functional annotations of the genes newly discovered as sequence-identified Ts-lethal alleles in this work, the number of identified genes in each class and examples of their land plant orthologs are shown.