Figure 4.

The ospm1 Knockout Mutant Created by CRISPR-Cas9 Also Shows a Drought-Sensitive Phenotype.

(A) The phenotype of the ospm1 mutant under drought stress. Thirty-five-day-old plants were drought-stressed for 7 d along with wild-type control plants. After being rewatered for 7 d, the plants were photographed.

(B) The survival rates of the ospm1 mutant and wild-type plants under drought stress. Results are means ± sd from three repeats, with 12 plants in each repeat. **P < 0.01 compared with wild-type control (Student’s t test; Supplemental Data Set 2).

(C) The gene editing sites of OsPM1 in the ospm1 mutant. The ORF of OsPM1 is shown in a purple frame. The sequence of the mutated OsPM1 is aligned with that of the wild-type OsPM1 gene. Magenta letters and dashes indicate the InDels introduced by gene editing.