Fig. 1.

The distribution of introns among strains of C. neoformans. In total there are 10 introns: two within the COB1 gene, five within the COX1 gene, one within the ND5 gene, and two within the LsrRNA gene. These fragments are amplified by primers flanking these introns. Lanes 1, 2, 3, 4 and 5 represent strains JEC21, YZX2, CHY618, CHY620 and H99 respectively. JEC21 is the model serotype D strain and H99 is the model serotype A strain. JEC21 and H99 are used here as intron positive and negative controls respectively and our results here confirmed the original description by Litter et al. (2005) and Toffalentii et al. (2004). Lane M is the marker 1 kb ladder. As can be seen from the gels, the MATa parental strain YZX2 differs from the MATα strains CHY618 and CHY620 at five introns: one located within ND5 and four located within the COX1 gene (Introns 1, 2, 4, and 5)