Table 2.
Primers for amplifying mtDNA fragments for genotyping in this study
| Primer pair name | Primer sequence (5’→3’) | Gene name and location of primer |
|---|---|---|
| ND2F | CAAGCTGCACCATTCCATA | ND2 |
| ND2R | CCATTAGTGGTGGTACTCC | ND2 |
| ND4F | GGGAGAATTTGATTCAAGTGCAAC | ND4 |
| ND4R | CATACATGGAAAGGTACTAG | ND4 |
| Da20 | GACACTACACAAGATGCCTC | COX1 exon 1 |
| Da3 | GCAATAGCATATACCATCCCG | COX1 exon 3 |
| Da22 | CTCGAGCTTACTTTACAGCAG | COX1 exon 3 |
| Da19 | GTACTACTCCTGTTAGTCCTC | COX1 exon 4 |
| Da26 | CAACGGCATACGGTGGATCTATCC | COX1 exon 4 |
| Da15 | CTGTTAGATATGATGGTGTGC | COX1 exon 6 |
| COB1F | CCACAACCTATTAACATTAGCTACGC | COB1 exon 1 |
| COB1R | CGTCTCCATCTACAAAGCCAGCAAAC | COB1 Intron 2 |
| ND5F | CTATTGGTGTTACAGGAGCTCAC | ND5 |
| ND5R | GAGCCTTCATACCTGCCTTATTTGC | ND5 |
| LsrRNAF | CAGCAGAACCCTTCCCAGC | Upstream LsrRNA |
| LsrRNAR | CCTCCACTGTCTCATGCGG | LsrRNA exon 3 |
| Cox1F | TGTCTGGAGCTGGTAACCAAT | COX1 exon 1 |
| Cox1R | AAGAGGTGTTCATATAGAACTGG | COX1 exon 1 |
| ATP6F | GACACACTTTATTACATCTCCAC | ATP6 |
| ATP6R | GAAGTTCAATGGCATCCTTG | ATP6 |
| N2A8F | AACTCCCCACATAGTTATGG | ND2-ATP8ig a |
| N2A8R | CATCCCTGTTATTAATTCACT | ND2-ATP8 ig |
| ATP8F | TTTCAATGGGTGCTGTGTTC | ATP8-COX1ig b |
| ATP8R | CCGAATGTAATTTGGTTTACCC | ATP8 |
| ATP9F | CGGACTATCAGGAGCTGGAG | ATP9 |
| ATP9R | TTGGTGGTCACCGTTTAGAA | ATP9-COX1ig c |
| ND6F | ACTTGATCTTCTTGCATTTGG | ND6 |
| ND6R | TTATGTTCGTGGTCGTAGACA | ND6 |
aND2-ATP8ig indicates that the primer is located in the intergenic region between ND2 and ATP8
bATP8-COX1ig indicates that the primer is located in the intergenic region between ATP8 and COX1
cATP9-COX1ig indicates that the primer is located in the intergenic region between ATP9 and COX1