Table 5.

Transfer frequency of ICEHs1, and phenotypic and genotypic characteristics of ICEHs1 donor (AVI 1 and AVI 31 strains), recipient (Rif^r H. somni AVI 14 and Rif^r P. multocida DSM 16031) and transconjugant strains.

Strains	ICEHs1 transfer frequency	Phenotype MIC (μg/mL)			Genotype
		tet	Cu	Zn	tet(H)	ebrB	mco	czcD	int	tnp
H. somni AVI 1	Donor	16	4	2	+	+	+	+	+	+
H. somni AVI 31	Donor	16	2	2	+	+	+	+	+	+
H. somni AVI 14 Rifr	Recipient	0.25	0.25	1	–	–	–	–	–	–
P. multocida DSM 16031 Rifr	Recipient	1	2	1	–	–	–	–	–	–
H. somni AVI 1 → AVI 14 Rifr	1.16 × 10−7	16	4	2	+	+	+	+	+	+
H. somni AVI 31 → AVI 14 Rifr	1.23 × 10−7	16	2	2	+	+	+	+	+	+
H. somni AVI 1 → P. multocida DSM 16031 Rifr	1.57 × 10−8	16	4	2	+	+	+	+	+	+

Rif^r, rifampicin resistant; tet, tetracycline; Cu, Copper; Zn, Zinc; tet(H), tetracycline resistance gene; ebrB, small multidrug efflux transporter gene; mco, Multicopper oxidase gene; czcD, cation efflux transporter gene; int, Integrase; tnp, transposase.