Table 2.
Escherichia coli strains used in this study
| Strains | Relevant characteristics | Source |
|---|---|---|
| Wild-type | E. coli K-12 MG1655 F− λ− ilvG- rfb-50 rph-1 | ATCC47076 |
| Keio strains | F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514 carrying single gene deletions | [54] |
| Sy62 | Δmdh::FRT | This study |
| Sy111 | Δmqo::FRT | This study |
| Sy68 | Δmdh::FRT Δmqo::FRT | This study |
| Sy102 | Δmdh::FRT Δmqo::FRT Δ(ackA-pta)::FRT | This study |
| Sy161 | Δmdh::FRT Δmqo::FRT Δ(ackA-pta)::FRT ΔmaeB::FRT | This study |
| Sy162 | Δmdh::FRT Δmqo::FRT Δ(ackA-pta)::FRT ΔmaeA::FRT | This study |
| Sy168 | Δmdh::FRT Δmqo::FRT Δ(ackA-pta)::FRT ΔmaeA::FRT ΔmaeB::FRT | This study |
| Sy249 | Sy68 expressing pACT3w-ppcK620S | This study |
| Sy242 | Sy102 expressing pACT3w-ppcK620S | This study |
| Sy252 | Sy161 expressing pACT3w-ppcK620S | This study |
| Sy254 | Sy162 expressing pACT3w-ppcK620S | This study |
| Sy280 | Sy168 expressing pACT3w-ppcK620S | This study |
| Sy279 | Sy168 expressing pACT3w-ppcwt | This study |
| Sy320 | Sy168 expressing pEXT20 s-ppcK620S | This study |
| Sy321 | Sy168 expressing pEXT20w-ppcK620S | This study |
| Sy322 | Sy168 expressing pACT3 s-ppcK620S | This study |
| Sy502 | Δmdh::FRT Δmqo::FRT ΔmaeA::FRT ΔmaeB::FRT expressing pACT3w-ppcK620S |
This study |
| Sy504 | Δmdh::FRT Δmqo::FRT ΔmaeA::FRT ΔmaeB::FRT ΔiclR::FRT expressing pACT3w-ppcK620S |
This study |
| Sy510 | Δmdh::FRT Δmqo::FRT ΔmaeA::FRT ΔmaeB::FRT ΔiclR::FRT ΔackA::FRT expressing pACT3w-ppcK620S |
This study |
| Sy512 | Δmdh::FRT Δmqo::FRT ΔmaeA::FRT ΔmaeB::FRT ΔiclR::FRT ΔackA::FRT ΔpoxB::FRT expressing pACT3w-ppcK620S |
This study |
| Sy729 | Δmdh::FRT Δmqo::FRT ΔmaeA::FRT ΔmaeB::FRT ΔiclR::FRT ΔarcA::FRT expressing pACT3w-ppcK620S |
This study |
| Sy731 | Δmdh::FRT Δmqo::FRT ΔmaeA::FRT ΔmaeB::FRT ΔiclR::FRT ΔarcA::FRT expressing pACT3w-ppcK620S-gltAR163L |
This study |
| Sy936 | Δmdh::FRT Δmqo::FRT ΔmaeA::FRT ΔmaeB::FRT ΔiclR::FRT ΔarcA::FRT Δedd-eda::FRT expressing pACT3w-ppcK620S-gltAR163L |
This study |
| Sy939 | Δmdh::FRT Δmqo::FRT ΔmaeA::FRT ΔmaeB::FRT ΔiclR::FRT ΔarcA::FRT Δedd-eda::FRT ΔaspA::FRT expressing pACT3w-ppcK620S-gltAR163L |
This study |