Table 5.
Effect of temperature and co-incubation period on Arabidopsis root transformation
| Duration in co-transformation plate (days) | Growth temperature (°C) | Transformation efficiency (Mean ± SD) |
|---|---|---|
| 3 | 25 | 72.05 ± 5.7a |
| 16 | 14.86 ± 3.4b | |
| 6 | 25 | 32.82 ± 9.2a |
| 16 | 6.40 ± 0.9b | |
| 9 | 25 | 13.56 ± 4.4a |
| 16 | 3.29 ± 1.5b | |
| 12 | 25 | 2.50 ± 2.4a |
| 16 | 0.49 ± 0.3a | |
| Duration in 16 °C Pr > F 0.0001 | ||
| Growth temperature (°C) Pr > F 0.0001 | ||
| Duration in 16 °C * Growth temperature(°C) Pr > F 0.0001 | ||
Each treatment consisted of five plates (each plate is a technical replicate) and the experiments were conducted three times (biological replicates). The efficiency values represent the average ± SD (n = 09). Significantly different transformation efficiencies are represented by different letters