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. 2018 May 21;2(7):861–871. doi: 10.1002/hep4.1185

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© 2018 The Authors. Hepatology Communications published by Wiley Periodicals, Inc., on behalf of the American Association for the Study of Liver Diseases.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Lin28B directly regulates AURKA through RAN. (A) qPCR (left) and western blot (right) analysis of Lin28B, RAN, and AURKA expression in HB cell lines HUH6, HepG2, and Hep293TT and normal hepatocyte cell line HC‐04. (B) qPCR analysis of LIN28B, RAN, and AURKA mRNA levels in HUH6, HepG2, and Hep293TT cells transfected with control, siLIN28B, or siRAN after 48 hours. LIN28B, RAN, and AURKA mRNA levels are normalized to β‐actin levels. *P < 0.05, **P < 0.01, ***P < 0.001. Data are shown as mean ± SEM (triplicate assays). (C) Western blot analysis of LIN28B, RAN, and AURKA protein expression in HUH6, HepG2, and Hep293TT cells transfected with control, siLIN28B, or siRAN for 2 days, with β‐actin serving as a loading control. Pooled samples (n = 3 per group) were run in a single lane. Numbers indicate density relative to siCon. Abbreviations: qPCR, quantitative polymerase chain reaction; siCon, siControl.