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. 2018 May 21;2(7):861–871. doi: 10.1002/hep4.1185

Figure 4.

Figure 4

Lin28B and AURKA are novel target genes of miR‐26a‐5p. (A) Target prediction for miR‐26a‐5p using Targetscan identified miR‐26a‐5p binding sequences within LIN28B 3′‐UTR and AURKA 3′‐UTR. (B) Luciferase reporter assays of LIN28B 3′‐UTR and AURKA 3′‐UTR in HUH6 and HepG2 cells transfected with 100 nmol miR‐26a‐5p. (C) Luciferase reporter assays of LIN28B 3′‐UTR mutant and AURKA 3′‐UTR mutant in HUH6 and HepG2 cells transfected with 100 nmol miR‐26a‐5p. (D) qPCR analysis of miR‐26a‐5p expression in normal hepatocyte cells HC‐04 and HB cell lines HUH6, HepG2, and Hep293TT. (E) Western blot analysis of LIN28B, RAN, and AURKA protein expression in the indicated cells transfected with mimic control and miR‐26a‐5p mimic for 48 hours, with β‐actin serving as a loading control. Numbers indicate density relative to control. (F) qPCR analysis of miR‐26a‐5p and miR‐21‐5p expression in normal liver (n = 16) and FFPE HB tissues (n = 14). All data are shown as mean ± SEM (triplicate assays). *P < 0.05 versus HC‐04. Abbreviations: CON, control; MUT, mutant; qPCR, quantitative polymerase chain reaction.