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. 2018 Jun 21;2(7):845–860. doi: 10.1002/hep4.1196

Figure 2.

Figure 2

Characterization of EC‐Wls‐KO reveals low liver weights and defective metabolic zonation. (A) EC‐Wls‐KO mice (n = 6) have significantly lower LW/BW ratio at baseline compared to littermate controls (n = 7). (B) Representative WB shows noteworthy decreases in Cyp2e1, GS, Cyp1a2, and active‐β‐catenin in EC‐Wls‐KO at baseline while total β‐catenin is unaltered. (C) Representative IHC shows the presence of GS, Cyp2e1, and Cyp1a2 in EC‐Wls‐CON in zone 3 hepatocytes and their absence in EC‐Wls‐KO (magnification × 50). (D) Significantly lower serum ALT and AST shows complete protection in EC‐Wls‐KO at 24 hours in response to a single intraperitoneal injection of 450 mg/kg of acetaminophen compared to EC‐Wls‐CON. (*P < 0.05; ***P < 0.001). Abbreviations: ALT, alanine aminotransferase; AST, aspartate aminotransferase; LW/BW, liver weight to body weight. In graphs, each data point represents one animal, and the mean and standard deviations are represented by the horizontal lines.