FIGURE 6.
Calculation of selectability (Δftemp) in simultaneously fed para-templating pools. (A) A representative pool history for a pool making GppG template internally, with labeled product concentrations. Note particularly stable nucleotides (e.g., pG), somewhat unstable nucleotides (e.g., AppA*), and unstable mixed activated nucleotides (e.g., ImpNt; 2-methyl-imidazolyl-5′-nucleotides). Note also nucleotide concentrations in pools receiving substrates randomly, but at a mean of 100 mM. (B) Means for 1000 pools like the one in A. Note particularly the integrated fraction of templated AppA* synthesis, ftemp, which is zero at pool initiation. Thus there are necessarily early times when templating is negligible with respect to untemplated, chemical product synthesis. (C) Mild and strong selection of a functional ribodinucleotide product, AppA*. Data are means of 1000 pools. (D) Response of fractional templated synthesis (ftemp) to selection for total product AppA*. The short dashed arrow is average Δftemp as a result of mild selection at 50 d; the longer dashed arrow is Δftemp as a result of strong selection at 50 d in 1000 pools. These quantities are also plotted in Figure 1J.