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. 2018 Aug;24(8):1080–1092. doi: 10.1261/rna.064345.117

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© 2018 Singh et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 1. — Steady-state (Michaelis–Menten) kinetics of the methyltransferase reaction catalyzed by wild-type _TkTrm10. Curves are shown using either SAM at a fixed concentration of 20 µM and varying concentrations of tRNA-G (A) or tRNA-A (B), or using SAM as a variable substrate at a fixed concentration (4 µM) of tRNA-G (C) or tRNA-A (D). Every data point is the average (±SEM) of three independent measurements. The k_cat and K_M values (±SE) resulting from fitting on the Michaelis–Menten equation are given in the insets.