Fig. 2.

Biofilm formation by B. cereus on different surfaces and at different temperatures. A biofilm was grown on stainless steel (SS), plastic slides (PS), or glass slides (GS) at 25 (A) or 30 °C (B) in TSB for 48 h and quantified using the CV assay. The data represents the average of two independent biological experiments, each performed in triplicates (n = 6), and the standard deviations. The threshold of biofilm formation (solid line) is equal to the background absorbance value plus three times the standard deviation (OD = 0.2). Groups with different alphabets within each strain indicate a significant difference (Tukey’s post hoc test p < 0.05). (asterisk) indicates a significant difference (p < 0.05) compared to incubation at 25 °C (t test, p < 0.05)