Fig. 4.

Biofilm formation by B. cereus in response to NaCl or a combination of glucose and NaCl. Strains A ATCC14579, B GIHE 62-5, C GIHE 62-9, and D GIHE 617-5 were grown on 24-well polystyrene microtiter plates in mTSB supplemented with different concentrations of NaCl, i.e., ranging from 0 to 8%, or in combination with glucose (0.25%) and NaCl; they were incubated at 30 °C for 48 h. Biofilm formation was quantified using the CV assay for absorbance at 595 nm. The data represents the average of three independent biological experiments, each performed in triplicates (n = 9), and the vertical bars indicate the standard deviations. Groups with different alphabets within each strain and each treatment indicate a significant difference (Tukey’s post hoc test, p < 0.05). Asterisk indicates a significant difference (p < 0.05) compared to NaCl