Fig. 2.
GLMOC inhibited the expression of COX-2 and prostaglandin E2 level in LPS-induced RAW264.7 cells. (A) The PGE2 amount in the media was determined by competitive ELISA. The cells (8 × 105 cells in a 60 mm dish) were pretreated with GLMOC or SA for 2 h, and then LPS for 22 h. (B) The relative amount of COX-2 mRNA was measured with quantitative RT-PCR. The experimental condition is same with Fig. 1(B). (C) The protein levels of COX-2 and β-actin were determined by Western blot. The experimental condition is same with Fig. 1(C). Values represent the mean ± SD. Different lowercase letters indicate significant differences at p < 0.05. GLMOC (50, 100, and 300 μg/mL), SA (10 μM), LPS (100 ng/mL)