Fig. 4.
GLMOC blocked the NF-κB signaling pathway in LPS-stimulated RAW264.7 cells. The cells (8 × 105 cells in a 60 mm dish) were pretreated 2 h with GLMOC or SA, and then LPS was treated for 30 min. The protein levels of phosphorylated p65 (p-p65) and p-65, IκB, and β-actin (A), and the nuclear protein levels of p-p65, p-65, and lamin B (B) were determined by Western blot. Values represent mean ± SD. Different lowercase letters indicate significant differences at p < 0.05. GLMOC [50, 100, and 300 μg/mL (A) or 300 μg/mL (B)], SA (10 μM), LPS (100 ng/mL)