Fig. 6.

GLMO is the major components exerting anti-inflammatory effect without cytotoxicity in RAW264.7 cells. (A) The cells (8 × 10⁵ cells in a 60 mm dish) were pretreated with GLMOC (300 μg/mL), GLMO (96.7 μg/mL), olive oil (OL, 193.5 μg/mL), and vitamin E (VE, 9.8 μg/mL) for 2 h, and then LPS (100 ng/mL) for 22 h. The protein levels of iNOS, COX-2, and β-actin were determined by Western blot. (B) The cells (1 × 10⁴ cells/well in 96-well plate) were treated with GLMOC (300 μg/mL), GLMO (96.7 μg/mL), olive oil (OL, 193.5 μg/mL), and vitamin E (VE, 9.8 μg/mL) for 24 h, and the cell viabilities were determined by MTT assay. Values represent the mean ± SD. Different lowercase letters indicate significant differences at p < 0.05