Fig. 4.

Modulation of subcellular localization of DAF-16 by NAC. (A) Subcellular localization of DAF-16 can be observed with the GFP transgene fused with the daf-16 gene. Worms are classified into three groups, “cytosolic,” “intermediate,” and “nuclear,” depending on the subcellular distribution of fluorescence. (B) In the worms pretreated with NAC, more GFP localizations into nuclei are observed compared to untreated control (n = 100). The error bar indicates standard error. Asterisk means the difference is statistically significant (p < 0.05)