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. 2018 Jul 4;5(3):ENEURO.0060-18.2018. doi: 10.1523/ENEURO.0060-18.2018

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Copyright © 2018 Ilinsky et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 1. — Illustration of consecutive stages of image processing. A, Example of nuclear outlines traced from a 50-µm-thick section. B, Example of a group of aligned outlines. C, A group of outlines with thickness added. D, Medial view of 3D-reconstructed volume of color-coded structures. Positions of coordinate planes are indicated with the two perpendiculars: horizontal axis indicates position of the intercommissural plane, i.e., zero horizontal plane, vertical axis shows the position of the zero frontal plane.