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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Nat Protoc. 2018 Apr 26;13(5):1106–1120. doi: 10.1038/nprot.2018.014

Figure 1. Schematic representation of the processes involving determination of bound lipids to membrane protein.

Figure 1

Overall workflow to identify membrane protein systems where lipid binding is critical for oligomeric stability. Following the native mass spectrum the HE-nMS platform is employed to identify the bound lipids through MS/MS of the isolated lipid-protein complex. In cases where the purpose of the experiment is to identify bound lipids, irrespective their role in maintaining the structural assembly, only the RHS of the diagram should be followed.