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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Nat Protoc. 2018 Apr 26;13(5):1106–1120. doi: 10.1038/nprot.2018.014

Figure 2. Delipidation of dimeric LeuT.

Figure 2

Progressive delipidation of LeuT dimer in 1% (wt/vol) delipidating detergent NG. a, Native MS of LeuT in OG shows dimer with 7.4 kDa lipid adduct (observed mass 126030 Da, expected mass of 119.8kDa). b, Progressive delipidation in NG detergent, monitored by native MS, indicates partial dissociation after 2h; however no apo dimer is observed and a 7.4kDa lipid adduct is retained with the dimer. Monomeric LeuT is devoid of lipid and the measured mass correlates well with the theoretical mass. c, Complete delipidation and dissociation of LeuT is achieved after 12h. Delipidation causes disintegration of the dimer into free monomers. Protein subunits are shown as grey and green spheres with lipids represented in red.The relative sizes of the protein and lipids are not to scale.