Table 2.
Probiotic characterization of Bacillus strains
| Treatment | Viable cell number (Log CFU/mL) | |||
|---|---|---|---|---|
| Vegetative cell | Spore cell | |||
| B. clausii ATCC 700160 | B. subtilis P223 | B. clausii ATCC 700160 | B. subtilis P223 | |
| Resistance to artificial gastric juice and bile salts | ||||
| Initial cell number | 6.82 ± 0.35a1 | 6.88 ± 0.18b | 5.69 ± 0.21b | 5.04 ± 0.57a |
| 0.3% (w/v) pepsin, pH 2.5, 3 h | ND2 | 4.15 ± 0.22a | 5.31 ± 0.16a | 5.38 ± 0.59a |
| 0.3% (w/v) oxgall, 24 h | 7.50 ± 0.49a | 7.14 ± 0.32b | 7.86 ± 0.10c | 7.07 ± 0.36b |
| Adhesion to HT-29 cell | ||||
| Initial cell number | 7.57 ± 0.78a | 6.96 ± 0.09a | 5.57 ± 0.08a | 4.63 ± 0.05a |
| Adhesion cell number | 5.00 ± 0.05b | 4.52 ± 0.09b | 4.39 ± 0.08b | 3.36 ± 0.10b |
| β-glucuronidase | – | – | NT3 | NT |
| Hemolysis | – | – | NT | NT |
| Toxin genes (hblA, hblC, hblD, nheA, nheB, nheC) |
– | – | NT | NT |
1a–c Different superscript letters in the same row indicate statistical differences in each characteristic (p < 0.05). Values are expressed as mean ± SD (n = 3)
2Not detected
3Not tested