Fig. 1.

Electropherograms of WGEHs produced in distilled water for 1 h at 50 °C by one- (A), two- (B), three- (C), and four-enzyme (D) hydrolysis at ambient pressure and 300 MPa. Lane description. (A): 1 molecular mass markers; 2 12% WG without enzyme treatment (ambient pressure); 3 F (ambient pressure); 4 F (300 MPa); 5 A (ambient pressure); 6 A (300 MPa); 7 P (ambient pressure); 8 P (300 MPa); 9 M (ambient pressure); 10 M (300 MPa). (B): 1 molecular mass markers; 2 12% WG without enzyme treatment (ambient pressure); 3 F–A (ambient pressure); 4 F–A (300 MPa); 5 F–P (ambient pressure); 6 F–P (300 MPa); 7 F–M (ambient pressure); 8 F–M (300 MPa); 9 12% WG without enzyme treatment (300 MPa); 10 molecular mass markers. (C): 1 molecular mass markers; 2 12% WG without enzyme treatment (ambient pressure); 3 F–A–P (ambient pressure); 4 F–A–P (300 MPa); 5 F–A–M (ambient pressure); 6 F–A–M (300 MPa); 7 F–P–M (ambient pressure); 8 F–P–M (300 MPa); 9 12% WG without enzyme treatment (300 MPa); 10 molecular mass markers. (D): 1 molecular mass markers; 2 12% WG without enzyme treatment (ambient pressure); 3 12% WG without enzyme treatment (300 MPa); 4 F–A–P–M (ambient pressure); 5 F–A–P–M (300 MPa). For all hydrolytic processes, F, A, P, and M denote Flavourzyme 500MG, Alcalase 2.4L, Protamex, and Marugoto E, respectively