Table 1.
Protease combination | TSS (%) | |
---|---|---|
Ambient pressure | 300 MPa | |
Blank1 | 4.89 ± 0.09hB | 6.16 ± 0.10jA |
F2 | 42.00 ± 0.18fB | 67.23 ± 0.24gA |
A | 25.58 ± 0.21gB | 43.65 ± 0.50iA |
P | 50.85 ± 0.36eA | 49.43 ± 0.33hB |
M | 49.48 ± 0.38eB | 74.08 ± 0.84fA |
F–A3 | 75.78 ± 3.15cA | 80.84 ± 1.15dA |
F–P | 76.06 ± 0.36cB | 81.06 ± 0.27dA |
F–M | 69.10 ± 0.88 dB | 78.05 ± 1.15eA |
F–A–P4 | 77.10 ± 0.68bcB | 83.04 ± 0.63cA |
F–A–M | 76.84 ± 0.65bcB | 84.32 ± 0.45bA |
F–P–M | 78.29 ± 0.79abB | 84.19 ± 0.26bA |
F–A–P–M5 | 79.37 ± 0.90aB | 89.75 ± 0.82aA |
For all hydrolytic processes, F, A, P, and M respectively denote Flavourzyme 500MG, Alcalase 2.4L, Protamex, and Marugoto E, while TSS means total soluble solids. Values of TSS are mean ± SD (n = 3). Means within the same column with different lowercase letters are significantly different at p < 0.05 by ANOVA. Means within the same row with different capital letters are significantly different at p < 0.05 by ANOVA
1Twelve percent WG suspension
2One-enzyme hydrolysis
3Two-enzyme hydrolysis
4Three-enzyme hydrolysis
5Four-enzyme hydrolysis