Table 2.
Changes in soluble nitrogen and DH of WGEHs according to protease combinations
| Protease combination | Ambient pressure | 300 MPa | ||||
|---|---|---|---|---|---|---|
| TWSN (%) | TCASN (%) | DH (%)1 | TWSN (%) | TCASN (%) | DH (%) | |
| Blank2 | 0.049 ± 0.006 dB | 0.030 ± 0.002 dB | 1.74 | 0.056 ± 0.003eA | 0.052 ± 0.003eA | 3.03 |
| F | 0.600 ± 0.012cB | 0.400 ± 0.002cB | 23.70 | 0.990 ± 0.004dA | 0.920 ± 0.011dA | 53.81 |
| F–A | 0.980 ± 0.060bA | 0.480 ± 0.007bB | 28.13 | 1.086 ± 0.029cA | 1.024 ± 0.017cA | 59.63 |
| F–A–M | 1.140 ± 0.034aB | 1.091 ± 0.012aB | 63.54 | 1.230 ± 0.023bA | 1.200 ± 0.013bA | 70.18 |
| F–A–P–M | 1.140 ± 0.037aB | 1.099 ± 0.013aB | 64.01 | 1.290 ± 0.018aA | 1.230 ± 0.016aA | 71.98 |
The content of TN in 12% WG suspension without enzyme treatment was 1.717 ± 0.011%. For all hydrolytic processes, F, A, P, and M respectively denote Flavourzyme 500MG, Alcalase 2.4L, Protamex, and Marugoto E, while TWSN, TCASN, and DH respectively mean total water-soluble nitrogen, trichloroacetic acid-soluble nitrogen, and degree of hydrolysis. Values of TWSN and TCASN are mean ± SD (n = 3). Means within the same column with different lowercase letters are significantly different at p < 0.05 by ANOVA. Means within the same row with different capital letters are significantly different at p < 0.05 by ANOVA
1TCASN/TN × 100
2Twelve percent WG suspensions that underwent pressure treatments without enzymes at ambient pressure and 300 MPa, respectively