Table 1.
Primers used for PCR amplification of tyrosine decarboxylase (TDC) gene and reference gene in Enterococcus faecium KCCM12118 (ATCC 19434)
| Gene | Primer | Sequence(5′–3′) | Base pairs | Accession no. | Reference |
|---|---|---|---|---|---|
| tdc a | F | GTGAATCATTGTCGAAAGAT | 1867 | KF195934 | This study |
| R | GCTTCGCTTGCCAATAATTGG | ||||
| q-tdc b | F | AGACCAAGTAATTCCAGTGCC | 116 | KF195934 | This study |
| R | CACCGACTACACCTAAGATTGG | ||||
| q-gap c | F | ATACGACACAACTCAAGGACG | 139 | NC017960 | This study |
| R | GATATCTACGCCTAGTTCGCC |
atdc specific primer used for reverse transcription PCR
btdc specific primer used for real time qPCR
cgap reference primer used for real time qPCR