Fig. 2.

Anaerobic crystallization setup and transfer. (A) Simple but effective setup to bring a setup crystallization plate into the glovebox for less oxygen-sensitive [Fe–S] proteins using pipetting robot located outside the glovebox. The protection plate was placed on top of a protein crystallization plate and clamped down with paper clips to prevent evaporation from the drops and reservoir during the vacuum pump evacuation cycle. (B) Oxygen-sensitive PcrAB crystal grown in the glovebox at −20°C. The brown crystal color shows the presence of intact [Fe–S] clusters.