Fig. 1.

The effect of several parameters on the refolding of recombinant Il-DyP4. a pH, b EDTA concentration, c hemin concentration, and d urea concentration were systematically examined. All reactions were performed with 1.2 mg mL⁻¹ protein in 50 mM phosphate buffer. The reactions took place in the dark for 24 h at 4 °C. The residual activity of Il-DyP4 was measured according to enzyme activity assay. Deviation values are standard deviations based on triplicate determinations