Table 2.

Kinetic parameters—K_m (μM), k_cat (s⁻¹), and k_cat/K_m (s⁻¹M⁻¹) of Il-DyP4 and Il-MnP6 for substrates oxidation obtained based on the optimal pH and temperature (°C)

	Il-DyP4				Il-MnP6
	pH	Temperature	Kinetic constants		pH	Temperature	Kinetic constants
H2Oa2	3.5	35	K m	163 ± 20.8	4.5	30	K m	194 ± 18
			k cat	(1.2 ± 0.08) × 104			k cat	879 ± 48
			kcat/Km	(7.6 ± 0.5) × 107			kcat/Km	(4.5 ± 0.3) × 106
ABTS	3.5	35	K m	62 ± 11	3.5	45	K m	83 ± 6.5
			k cat	8356 ± 747			k cat	245 ± 7
			kcat/Km	(1.3 ± 0.1) × 108			kcat/Km	(3.0 ± 0.09) × 106
Mn2+	4.5	35	K m	2687 ± 463	4.5	30	K m	129 ± 8
			k cat	806 ± 94			k cat	369 ± 6.1
			kcat/Km	(3.0 ± 0.4) × 105			kcat/Km	(2.9 ± 0.3) × 106
DMP	4	35	K m	58 ± 3	4.5	55	K m	53 ± 8.6
			k cat	4896 ± 131			k cat	989 ± 100
			kcat/Km	(8.4 ± 0.2) × 107			kcat/Km	(1.9 ± 0.2) × 107
Guaiacol	4	45	K m	24 ± 7.5	4	45	K m	2.6 ± 0.6
			k cat	413 ± 34			k cat	184 ± 7
			kcat/Km	(1.7 ± 0.1) × 107			kcat/Km	(7.1 ± 0.3) × 107
VA	3.5	45	K m	(2.1 ± 0.98) × 104	–b	–	K m	–c
			k cat	108 ± 47			k cat	–
			kcat/Km	(5.2 ± 2.3) × 103			kcat/Km	–
RBlue 19	4.5	35	K m	133 ± 34	3	35	K m	81 ± 39.5
			k cat	5345 ± 921			k cat	7.3 ± 2.6
			kcat/Km	(4.0 ± 0.7) × 107			kcat/Km	(9.0 ± 3.2) × 104
RBlack 5	4	35	K m	159 ± 61	3	35	K m	4.4 ± 0.7
			k cat	267 ± 96			k cat	0.06 ± 0.003
			kcat/Km	(1.7 ± 0.6) × 106			kcat/Km	(1.5 ± 0.07) × 104

Means and 95% confidence limits

^aActivities were measured with 1.25 mM ABTS and 1 mM Mn²⁺ by Il-DyP4 and Il-MnP6, respectively

^bNot detected

^cExperimental condition: [VA] = 1 mM, [MnP] = 25 nM, [H₂O₂] = 0.1 mM, [MnSO₄] = 1.0 mM, [Tartrate buffer] = 100 mM, pH = 3.5, temperature = 45 °C, reaction time = 60 min