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. 2018 Jul 3;9(51):29619–29633. doi: 10.18632/oncotarget.25651

Figure 2. PCP4/PEP19 knockdown and overexpression in MCF-7 and SK-BR-3 cells.

Figure 2

(A) PCP4/PEP19 knockdown by siRNA transfection (PCP4 siRNA + as open column) resulted in markedly decreased PCP4/PEP19 mRNA expression in MCF-7 and SK-BR-3 cells. (B) In the same knockdown condition, protein expression of PCP4/PEP19 was significantly decreased in both cell lines. (C and D) PCP4/PEP19 expressing plasmid transfection (PCP4 plasmid + as open column) showed a significantly increased expression levels of PCP4/PEP19 mRNA (C) and protein (D) in MCF-7 and SK-BR-3 cells. Protein expression levels of PCP4/PEP19 were normalized by those of β-actin. *p<0.05, ** p<0.01 and *** p<0.001.