Figure 4. TMZ inhibits MGMT expression and induces p21 and cytochrome C expression in breast cancer cells.
MCF7 cells were plated overnight and subsequently treated with TMZ (644μM) on an every other day basis with harvest time points at 2, 4 and 6 days. TMZ induced MGMT expression inhibition was significant at 4 days (A). MCF7 cells were treated with different concentrations of TMZ (257μM to 772μM) on an every other day basis before harvest at 4 days. TMZ induced MGMT inhibitory effect correlated with increased cyt C and p21 expression (B).