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. 2018 Jul 3;9(51):29727–29742. doi: 10.18632/oncotarget.25696

Figure 7.

Figure 7

(A and B) BG+/-TMZ therapy decrease MGMT, ki-67 and p-TOP2A, p-AURKB, p-CDC2, p-CDC20 and induce p21 in breast tumors derived from mice. Representative samples (40X) are shown. IHC correlative quantified expression analysis was done using ImageJ (NIH) / ImmunoRatio plugin, as described in the methods section. The sections were immunostained for expression of MGMT, p53, p21 and ki-67. Tumors from mice treated with BG +/- TMZ had a significant decrease in the expression of MGMT and ki-67. p53 expression was not significantly altered in these treatment groups. Expression of p21 was significantly increased in all treatment groups compared to controls (A). BG+/-TMZ also decreased phospho-proteins CDC2, TOP2A, AURKB, KIF20A and CDC20 expression in breast tumors. Temozolomide alone decreased phospho-TOP2A and phospho-AURKB expression in the breast tumors (B) correlating with the in-vitro expression results (B).