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. 2018 Jul 17;13(7):e0200705. doi: 10.1371/journal.pone.0200705

Fig 2. Effects of OGD on the morphology, cell apoptosis, and viability of PC12 cells.

Fig 2

PC12 cells were cultured under OGD for 0, 1, 2, 4, 6, 8, 10, or 12 h and then cultured under normal conditions for 24 h. Cell morphology, apoptosis, and viability were evaluated. (A) PC12 cell morphology. OGD-induced PC12 cells exhibited round, slender, and degenerated morphologies. (B) Cell apoptosis. In the 2-h-OGD-induced group, early apoptotic cells emitted yellow–green fluorescence. In the 6-h-OGD-induced group, late apoptotic cells with concentrated, localized orange and red nuclear staining were detected. (C) Cell viability decreased with the length of OGD induction. CON, control group. *P < 0.05 compared with the control (0 h) group. **P < 0.01 compared with the control (0 h) group.