Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul;59(8):3608–3618. doi: 10.1167/iovs.18-24101

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright 2018 The Authors

This work is licensed under a Creative Commons Attribution 4.0 International License.

PMC Copyright notice

qRT-PCR and ELISA verification of the suppression of VEGFA by ascorbate. (A) The inhibition of ascorbate on VEGFA transcription was validated in a second batch of ARPE-19 cells treated with ascorbate (50 μM). (B) The transcription of Vegfa (homologue of VEGFA) in RPE-J cells was inhibited by ascorbate (50 μM) treatment. (C) ELISA results show that ascorbate time-dependently decreases the levels of VEGF protein in the conditioned culture medium. (D) ELISA testing shows that ascorbate dose-dependently decreases the levels of VEGF in the conditioned culture medium (***P < 0.001).