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. Author manuscript; available in PMC: 2018 Jul 17.
Published in final edited form as: Vis Neurosci. 2017 Jan;34:E011. doi: 10.1017/S0952523817000050

Figure 3. Caudal medial pulvinar (Pcm) cells express calretinin (CR) and neurokinin 1 (NK1) and align with bilateral superior colliculus (SC) projections.

Figure 3

Confocal images illustrate ipsilateral (A, C, green) and contralateral (D, F, green) projections to the pulvinar that were labeled by a unilateral virus injection in the SC. These sections were also stained with antibodies against CR (B, E, purple) to define the Pcm (which contains CR) and the lateral pulvinar (Pl, which does not contain CR). Adjacent sections (C, F) stained for CR (purple) and NK1 (green) illustrate that CR-positive Pcm cells express NK1. This expression pattern is shown at higher magnification in half micron optical sections in panels G (CR, purple), H (NK1, green) and I (CR, purple, and NK1, green, asterisks indicate cells labeled with both antibodies). Scale in A = 50 μm and applies to A–F. Scale in G = 10 μm and applies to G–I. dLGN, dorsal lateral geniculate nucleus, OT, optic tract, PT, pretectum. Methods as in Bickford et al. (2015) and Masterson et al. (2010).