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. 2018 Jul 17;7:e35224. doi: 10.7554/eLife.35224

Figure 7. Hydrotropic Solubilization Contributes to Nucleolar Protein Dynamics.

Figure 7.

(A) Oocyte nuclei were isolated from RFP-Fbl and GFP-Npm1 expressing oocytes and immediately transferred into ISB alone (top) or ISB supplemented with ATP, AMP-PNP, or both nucleotides and imaged. (B) When compared to untreated controls, maximum fluorescence intensity measurements for GFP-Npm1 and C) RFP-Fbl reveals loss of signal intensity after 1 mM ATP + 3 mM AMP-PNP or 4 mM ATP supplementation. Images in (A) are representative of 3 independent experiments in which maximum fluorescence intensity of GFP-Npm1 (B) and RFP-Fbl (C) from 155 to 264 nucleoli was quantified, pooled, and analyzed.