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. 2018 Jul 17;7:e35216. doi: 10.7554/eLife.35216

Figure 5. Perturbation of acinar cell fate is not reversed upon Arid1a restoration.

Figure 5.

(A) Schematic of dox administration timepoints for Arid1a restoration. (B) Gross morphology and mKate2 fluorescence of KC-RIK-shRen and KC-RIK-shArid1a pancreata on dox and after 4 weeks of dox withdrawal. (C) Immunofluorescence staining of C-RIK-shRen and –shArid1a tissue for Arid1a and the ductal marker Keratin 8. (D) H and E staining of KC-RIK-shRen and –shArid1a mice on dox and after 4 weeks of dox withdrawal. (E) Quantification of PanIN lesions per field in (D) measured by veterinary pathologist. No significant difference between KC-RIK-shArid1a (6421) mice on dox and upon dox withdrawal. (F) Representative immunohistochemistry staining for the ductal marker Sox9 in pancreata from KC-RIK-shRen and –shArid1a.6421 mice from same cohort quantified in (E) Alcian blue counterstain stains acidic mucins in mucinous lesions. Scalebars 100 μm.