Figure 3. In vivo photocrosslinking characterization of randomly generated EcFtsZ pBpa variants.

(A) Schematic illustrating the random in vivo photocrosslinking screening strategy for unbiased identification of amino acid positions mediating subunit interactions. (B) Immunoblotting analysis of the photocrosslinked products in lysates of E. coli LY928-ΔftsZ cells that were transformed with the library of plasmids within which the in-frame TAG amber codon was randomly inserted throughout the ftsZ gene, as probed with antibodies against EcFtsZ.