Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Gene (Escherichia coli)	FtsZ	NA	Uniprot ID: P0A9A6
Gene (Mycobacterium tuberculosis)	FtsZ	NA	Uniprot ID: P9WN95
Gene (Branchiostoma lanceolatum)	mNeonGreen	PMID:23524392	Uniprot ID: A0A1S4NYF2
Stain, strain background (Escherichia coli)	W3110 ftsZ::kan recA56 ΔftsZ	PMID:2045370		JKD7-1/pKD3, ftsZ conditional null strain with pKD3 rescue plasmid
Stain, strain background (Escherichia coli)	BW25113 ΔinsH11:: pBpa-tRNA synthetase /tRNA-pBpa	PMID:27298319
Stain, strain background (Escherichia coli)	BW25113 ΔinsH11:: pBpa-tRNA synthetase /tRNA-pBpa ΔftsZ	This paper		ftsZ conditional null strain with pBpa incorporation system
Stain, strain background (Escherichia coli)	BW25113 ΔinsH11::pBpa- tRNA synthetase/tRNA- pBpa ftsZ::ftsZ-AviTag	This paper		The endogenous ftsZ gene was modified to encode FtsZ linked with an AviTag at the C-terminus
Antibody	Rabbit anti-EcFtsZ polyclonal antibody	This paper		The antibody was harvested by immuning rabbit with the purified EcFtsZ protein. Dilution: 1:10000, for western blot
Antibody	Alkaline phosphatase- conjugated streptavidin	Beyotime	A0312	Dilution: 1:5000, for western blot
Recombinant DNA reagent	pJSB100	PMID:12896999		pJSB2-FtsZ
Recombinant DNA reagent	pTet-FtsZ	This paper		bla pBR322 P tet::ftsZ, P tet incates Tet-on/Tet-off promoter and the expression of FtsZ in this plasmid via leaky expression
Recombinant DNA reagent	pTac-L3-FtsZ-mNeonGreen	This paper		bla pBR322 P L3::ftsZ-mNeonGreen, P L3 incates a synthetic constitutive promoter selected from Anderson promoter collection
Recombinant DNA reagent	pTac-0.16-FtsZ-TAG- mNeonGreen	This paper		bla pBR322 P0.16::ftsZ-TAG- mNeonGreen, P0.16 indicates a synthetic constitutive promoter selected from Anderson promoter collection and TAG amber condon was inserted into FtsZ-TAG- mNeonGreen fusion protein
Chemical compound, drug	p-benzoyl-L-phenylalanine (pBpa)	Bachem	F-2800