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. Author manuscript; available in PMC: 2018 Jul 17.
Published in final edited form as: Dev Biol. 2011 Dec 13;362(2):194–218. doi: 10.1016/j.ydbio.2011.12.009

Fig. 19.

Fig. 19

Isolated epithelium from adult heterozygous control Atoh1fl/+;Rosa26CreER/+ and homozygous Atoh1fl/fl;Rosa26CreER/+ mice 6 days after initiation of Tamoxifen treatment. (A) The numerous Alcian Blue 8GX positive mucous cells in control epithelium (arrows) were largely absent from Atoh1 deleted (Atoh1fl/fl;Rosa26CreER/+) epithelium (B). (C) Similarly, Insm1+ enteroendocrine cells found in control epithelium (arrowheads) were largely absent from Atoh1 deleted epithelium (D). In contrast, the Dclk1+ Insm1− brush cell population increased dramatically in homozygous epithelium (D) relative to heterozygous control (C). (E, F) The brush cells in Atoh1-deleted epithelium appear normal and stain with the standard brush cell markers including Dclk1, UEA-I, and Gfi1b (see also the enlargement of the region boxed in D).