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. Author manuscript; available in PMC: 2018 Jul 17.
Published in final edited form as: Dev Biol. 2011 Dec 13;362(2):194–218. doi: 10.1016/j.ydbio.2011.12.009

Fig. 3.

Fig. 3

Optical sections of villus epithelium from CD-1 mice demonstrating the necessity of supplementing the existing brush cell markers with an enteroendocrine cell specific maker such as Insm1 to reliably distinguish enteroendocrine from brush lineage cells. (A) UEA-I and anti-Dclk1are not specific for brush cells because a subset of Insm1+ enter-oendocrine cells (arrow heads) also stain. (B) Brush cells (boxed) are UEA-I+ Dclk1+ but Insm1−, in contrast to Insm1+ enteroendocrine cells (arrow heads). DIC helps confirm brush cell identification.