FIG. 3.

Time-lapse image series from confocal movie of a Xenopus embryonic epithelium expressing fluorescent tubulin (green) and fluorescent histone H2B (red). The top row shows a metaphase spindle in a control epithelial cell; the spindle undergoes a characteristic series of rotations and movements to and from the cortex before undergoing anaphase at 02:18. The bottom row shows a metaphase spindle in an epithelial cell depleted of myosin-10 via morpholino injection. The spindle is initially normal but becomes abnormally long and undergoes pole fragmentation. The metaphase–anaphase transition is greatly delayed, and when it happens (at 27:24), highly abnormal. Time in min:s.