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. Author manuscript; available in PMC: 2018 Oct 25.
Published in final edited form as: Methods Cell Biol. 2018 Apr 25;144:259–285. doi: 10.1016/bs.mcb.2018.03.013

FIG. 8.

FIG. 8

(A) Schematic showing how to mount a sample on a glass slide. First, a ring of vacuum grease is applied to the slide. Then, a sample is mounted in a droplet of media to the center of the ring and oriented such that the animal hemisphere is facing up. Lastly, a coverslip is lowered on top of the sample and grease ring. (B) Schematic showing how to mount a sample on a metal slide. First, a ring of vacuum grease is applied around the center hole. Then, a coverslip is pressed into the grease ring, and the slide is flipped over. A second ring of vacuum grease is applied, and the sample is then mounted in the center in a droplet of media, animal hemisphere facing up. Lastly, a coverslip is lowered on top of the sample and grease ring.