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. Author manuscript; available in PMC: 2019 Jul 15.
Published in final edited form as: Cancer Res. 2018 May 16;78(14):3865–3876. doi: 10.1158/0008-5472.CAN-18-0056

Figure 5.

Figure 5

Effects of RAW264.7 osteoclast-conditioned medium and MLO-A5 osteocyte-conditioned medium on 4T1.2 spheroids in the presence and absence of Fluphenazine or Trifluoperazine. Of note, CN = control, FP = Fluphenazine, and TFP = Trifluoperazine. The single and double asterisks indicate p < 0.05 and p < 0.01, respectively. (A&B) Alteration in 3D cell viability of 4T1.2 spheroids by the conditioned medium from RAW264.7 cells. (C) Upregulation of Snail protein by RAW264.7 conditioned medium. (D) Roughness of 4T1.2 spheroids in response to Fluphenazine or Trifluoperazine. (E) 3D cell viability and relative proliferation of 4T1.2 spheroids in response to Fluphenazine or Trifluoperazine. (F-H) Alterations in Snail and p-Akt in response to MLO-A5 conditioned medium, Fluphenazine, and/or Trifluoperazine. (I) Interactions of 4T1.2 cells (green) and MLO-A5 cells (red). These two types of cells completely mixed. (J) Shrinkage of 4T1.2 spheroids (red) by 20 μM Fluphenazine or Trifluoperazine in the presence of MLO-A5 spheroids (green). The size of 4T1.2 spheroids (red) was significantly reduced by Fluphenazine or Trifluoperazine.