Skip to main content
. Author manuscript; available in PMC: 2019 Jul 15.
Published in final edited form as: Cancer Res. 2018 Jun 29;78(14):3877–3887. doi: 10.1158/0008-5472.CAN-17-2892

Figure 1.

Figure 1

Loss of CypA results in delayed mammary morphogenesis. (a) Mammary gland whole mounts were dissected from CypA+/+ and −/− mice at the times indicated and fixed, processed, and stained with carmine alum prior to microscopy (Bar = 750 microns). (b) Paraffin-embedded inguinal mammary glands dissected at the indicated times were thin sectioned and stained with hematoxylin and eosin. (Bar = 250 microns). (c) The number of alveolar buds per mm of duct length was quantitated in CypA+/+ and −/− females at 14 weeks of development in whole mount mammary preparations (n = 4). (d) Terminal end buds were manually counted at the growth front into mammary adipose tissue in CypA+/+ and −/− mice at 14 weeks of development in whole mount mammary preparations (n = 5 each). (e) The maximal ductal outgrowth in mm from the reference point of the intra-mammary lymph node was measured at 14 weeks of age in whole mount mammary preparations from CypA+/+ and −/− mice (n = 5 each).