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. Author manuscript; available in PMC: 2019 Jul 17.
Published in final edited form as: Biochemistry. 2018 Jul 3;57(28):4125–4134. doi: 10.1021/acs.biochem.8b00552

Table 1.

Kinetic parameters of EntF with YbdZ variantsa.

Protein: Kd (nM) Km (uM Ser) Kcat(s−1) Kcat/Km(M−1s−1)
EntF - 1029 ± 171 0.84 ± 0.050 816
EntF + WT YbdZ 186 ± 69 116 ± 22 0.88 ± 0.04 7858
EntF + D8A YbdZ 555 ± 150 279 ± 59 0.97 ± 0.08 3476
EntF + I16A YbdZ 109 ± 27 430 ± 83 1.26 ± 0.11 2930
EntF + L17A YbdZ 295 ± 88 159 ± 32 0.93 ± 0.05 5849
EntF + W27A YbdZ 636 ± 78 1013 ± 143 0.98 ± 0.07 848
EntF + W37A YbdZ 1177 ± 338 438 ± 130 1.1 ± 0.11 2255
EntF + S48A YbdZ 41 ± 10 451 ± 59 1.14 ± 0.72 2527
EntF + L53A YbdZ 213 ±100 453 ± 92 1.29 ± 0.13 2847
EntF + A55S YbdZ 270 ± 88 450 ± 80 1.01 ± 0.08 2244
EntF + P62A YbdZ 229 ± 85 297 ± 60 1.21 ± 0.10 4074
EntF + Q69A YbdZ 374 ± 130 208 ± 74 0.94 ± 0.11 4519
EntF/P6A - 382 ± 59 0.93 ± 0.04 2434
EntF/I16A - 129 ± 34 0.78 ± 0.04 5736
EntF + MbtH10 54.6 ± 8 229 ± 31 0.74 ± 0.04 3231
EntF + PA241210 358 ± 95 397 ± 94 1.21 ± 0.06 3047
a

Data shown in graphical form in Figs. S5 and S6.