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. Author manuscript; available in PMC: 2019 Jul 15.
Published in final edited form as: Cancer Res. 2018 May 15;78(14):4022–4035. doi: 10.1158/0008-5472.CAN-17-3728

Figure 3.

Figure 3

Multiple DnaJ isoforms interact with AR and ARv7.

(A) Lysate from HEK293 cells transfected with FLAG-ARv7 or FLAG-AR was probed with FLAG-beads. Bound proteins were detected by WB.

(B) Lysate from HEK293 cells cotransfected with indicated FLAG-DnaJ family members and GFP-AR or GFP-ARv7 was probed with FLAG-beads. Bound proteins were detected by WB. Red arrow notes detection of native FLAG-DnaJC5, which tends to oligomerize. AR antibody N-20 was used to detect FL-AR and ARv7.

(C) Lysate from 22Rv1 CRPC cells transfected with indicated FLAG-DnaJ family members was incubated with FLAG-beads. Bound proteins were detected by WB. Red arrow notes detection of native FLAG-DnaJC5, which tends to oligomerize.

Experiments were repeated at least three times.