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. Author manuscript; available in PMC: 2019 Jul 15.
Published in final edited form as: Cancer Res. 2018 May 15;78(14):4022–4035. doi: 10.1158/0008-5472.CAN-17-3728

Figure 6.

Figure 6

Hsp40 and Hsp70 inhibition destabilizes GR in CRPC cells.

(A, B) WB of cell lysate from 22Rv1 or VCaP CRPC cells treated with increasing concentrations of C86 (A, left, right, respectively) or JG98 (B, left, right, respectively) for 6 hours. Red arrows note detection of HMW GR species.

(C) Biotin-C86 binding profile from 22Rv1 CRPC cells incubated with excess unlabeled vehicle (DMSO) or 50 μM C86 for 1 hour as assessed by streptavidin-bead IP and WB.

(D) GRE-(left) or LacZ Control-Reporter (middle) assay of yeast cells treated with vehicle or C86 as detected by chemiluminescence. Absorbance at O.D. 600 was utilized to measure yeast viability +/− C86 (right).

Experiments were repeated at least three times.